绱�(x矛)鑳炲収(n猫i)澶�pH鐨勮畩鍖栧湪鐢熺悊鍜岀梾鐞嗛亷绋嬩腑鎵紨钁楀緢閲嶈瑙掕壊锛岀窗(x矛)鑳炲収(n猫i)pH鐨勮畩鍖栨渻(hu矛)瀹规槗瑾樺皫(d菐o)绱�(x矛)鑳炲垎瑁�銆佸揩閫熺敓闀�(zh菐ng)鍜屽姛鑳界殑澶辫(di脿o)銆傜窗(x矛)鑳炲pH璁婂皬鍛堥吀鎬ф槸鐧岃畩浠ュ強鍏跺畠鐥呰畩鐨勪竴鍊�(g猫)閲嶈淇¤櫉(h脿o)銆傚皪(du矛)绱�(x矛)鑳炲収(n猫i)澶栦笉鍚屽崁(q奴)鍩�pH鐨勬娓�(c猫)鏈夊姪浜庡皪(du矛)绱�(x矛)鑳炵敓鍛介亷绋嬩互鍙婄梾鐞嗘(j墨)鍒剁殑鐮旂┒銆�
ANNA鏄竴鍊�(g猫)鐞嗘兂鐨勬瘮鐜囧瀷pH鐔掑厜鎺㈤嚌:
l 闈堟晱搴﹂珮锛�ANNA鐨勫惛鏀跺拰鐧�(f膩)灏勫厜璀滃湪鐢熺悊pH鑼冨湇鍏�(n猫i)锛�pH 6-8锛夛紝骞朵笖鍦ㄩ吀鎬у拰鍫挎�х媭鎱�(t脿i)涓嬮兘鏈夊緢楂樼殑鐔掑厜閲忓瓙鐢�(ch菐n)鐜囷紱
l 鍙嶆噳(y墨ng)鎬уソ锛�ANNA鐨勭晶鍩哄彲浠ュ伓鑱�(li谩n)鍏跺畠鍚勭ó鐢熺墿鍒嗗瓙銆佽棩鐗╁垎瀛愭垨寰磵鏉愭枡锛岃�屽叾pH闊挎噳(y墨ng)鍔熻兘涓嶅彈褰遍熆銆傚洜?y脿n)妞�?/span>ANNA鐨�pH闊挎噳(y墨ng)鏄締鑷簬4H-1,2,4-涓夋爱鍞�(1,5-a)浜屾爱闆滅挵(hu谩n)-1,8-钀橀叞浜炶兒绲�(ji茅)妲�(g貌u)锛屼笖鐔掑厜淇¤櫉(h脿o)涓嶉毃閰颁簽鑳�N-鍙栦唬鍩虹殑鏀硅畩鑰屾敼璁�锛�
l 鐢�(ch菐n)鍝佺┅(w臎n)瀹氾細灏�(du矛)pH闊挎噳(y墨ng)鍏锋湁鑹ソ鐨勫彲閫嗘�э紝鍙互闀�(zh菐ng)鏅�(sh铆)闁撳弽寰�(f霉)浣跨敤锛屽皪(du矛)鍏夈�佺┖姘ｇ瓑闈炲父绌�(w臎n)瀹�锛�
l 鎶楀共鎿捐兘鍔涘挤(qi谩ng)锛岄仼鐢ㄨ寖鍦嶅唬锛氶洟瀛愬挤(qi谩ng)搴﹀皪(du矛)鐔掑厜鍦�(tu谩n)涓嶇敘(ch菐n)鐢熷共鎿�锛屽彲鐢ㄤ簬绱�(x矛)鑳炪�佸嫊(d貌ng)鐗╁京(f霉)闆滅敓鐗╅珨绯�銆�[1-3]
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝�鍚嶇ū
|
绨�(ji菐n)绋�
|
CAS
|
绲�(ji茅)妲�(g貌u)寮�
|
|
2668726
|
N-宸遍吀鍩�-4H-1,2,4-涓夋爱鍞�(1,5-a)浜屾爱闆滅挵(hu谩n)-1,8-钀橀叞浜炶兒
3,9-Dihydro-1,3-dioxopyrido[3,4,5-gh][1,2,4]triazolo[1,5-a]perimidine-2(1h)-hexanoic acid
|
ANNA
|
1359819-97-8
|
|
n 鐢�(ch菐n)鍝佷娇鐢ㄨ鏄�
1锛夌窗(x矛)鑳炲湪鎴愬儚鐨勫墠缃簬鐩村緫鐐�20mm鐨勭幓鐠冨熀搴曠窗(x矛)鑳炲煿椁�(y菐ng)鐨匡紙MatTek Co.锛夊煿椁�(y菐ng)24h銆�
2锛夊皣pH鎺㈤嚌ANNA锛�4 mM锛夎垏绱�(x矛)鑳炲湪绱�(x矛)鑳炲煿椁�(y菐ng)鍩轰腑瀛佃偛1-2h銆�
3锛夌敤PBS绶╂矕娑诧紙pH=7.4锛夋礂绱�(x矛)鑳炰笁娆�锛屽幓闄ゆ父闆㈢殑鎺㈤嚌銆�
4锛夌窗(x矛)鑳炴垚鍍忕敤100鍊嶅叡鑱氱劍椤井閺¤瀵�銆傛縺鐧�(f膩)娉㈤暦(zh菐ng)鏄�405nm鍜�488nm锛岀啋鍏変俊铏�(h脿o)鏀堕泦1閫氶亾425-475 nm鍜�2閫氶亾500-600 nm鐨勭啋鍏夊挤(qi谩ng)搴�銆傦紙濡傞厤鏈�455nm婵�鍏夊櫒锛氬彲鐩存帴鐢�455nm婵�鐧�(f膩)锛屾敹闆�1閫氶亾锛�470nm-500nm锛夊拰2閫氶亾锛�505nm-540nm锛夌啋鍏夊挤(qi谩ng)搴︼級
5锛�pH妯�(bi膩o)婧�(zh菙n)鏇茬窔锛氱敤1mL鍚�5 ug /mL nigericin锛堜竴绋亱(y霉n)杓夐墍闆㈠瓙鍜屾矮闆㈠瓙鐨勮級楂�锛岃兘澶犱娇绱�(x矛)鑳炲収(n猫i)澶栫殑pH蹇�熺殑閬�(d谩)鍒颁竴鑷达級鐨勪笉鍚�pH鐨勯珮K+ HEPES绶╂矕娑茶檿鐞嗙窗(x矛)鑳�10min銆� 楂�K+ HEPES绶╂矕娑插惈鏈�125 mM KCl, 20 mM NaCl, 0.5 mM CaCl2, 0.5 mM MgCl2, 5 mM 钁¤悇绯栧拰20 mM HEPES銆傜劧鍚庨��(j矛n)琛岀窗(x矛)鑳炴垚鍍�锛� 鏍规摎(j霉)I2閫氶亾/I1閫氶亾鑸�pH鐨勯棞(gu膩n)绯荤躬鍒舵洸绶�锛屽皣寰呮脯(c猫)妯ｅ搧姣斿�煎付鍏ユ(bi膩o)婧�(zh菙n)鏇茬窔锛岀嵅寰楀(sh铆)闅涚窗(x矛)鑳炲収(n猫i)澶栫殑pH銆�
n 鏇村鐔掑厜鎺㈤嚌鐢�(ch菐n)鍝�
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝�鍚嶇ū
|
CAS
|
绲�(ji茅)妲�(g貌u)寮�
|
|
2668725
|
2,6-闆欙紙2-锛�3,3-浜岀敳鍩�-1-涓欏熀-浜屾矮鍚插摎-2-浜炲熀锛変簽涔欏熀锛�-鐠�(hu谩n)宸遍叜
2,6-Bi(2-(3,3-dimethyl-1-propylindolin-2-ylidene)ethylidene)-cyclohexanone
|
1442645-34-2
|
.bmp)
|
|
2668724
|
2,9-闆�[4-锛�4-鐢插熀鍝屽棯-1-鍩猴級鑻箼鐑熀]-1,10-鑿插挴鍟�
2,9-Bis[4-(4-methylpi-perazin-1-yl)styryl]-1,10-phenanthroline
|
1792998-33-4
|
.bmp)
|
|
2668727
|
CSTS
|
N/A
|
.bmp)
|
|
433521
|
D-鐔掑厜绱犻墍楣�
D-Luciferin potassium salt
|
115144-35-9
|
.bmp)
|
|
102273
|
2,3-浜屾皑鍩鸿悩
2,3-Diaminonaphthalene
|
771-97-1
|
.bmp)
|
|
2668723
|
9-鐢插熀-3,6-闆�[5-锛�4-鐢插熀鍝屽棯-1-鍩猴級-1H-鑻苟[d]鍜攽-2-鍩�] -9H-鍜斿攽
9-Methyl-3,6-bis[5-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl]-9H-carbazole
|
1514901-89-3
|
.bmp)
|
|
227585
|
閯拌嫰浜岀敳閱�
o-Phthalaldehyde
|
643-79-8
|
.bmp)
|
n 鐩搁棞(gu膩n)鏂囩嵒(xi脿n)
[1] Zhou, J., Fang, C.L., Chang, T.J., Liu, X.J. and Shangguan, D. A pH sensitive ratiometric fluorophore and its application for monitoring the intracellular and extracellular pHs simultaneously. J Mater Chem B, 1, 661-667 (2013).
[2] Hou, Y., Zhou, J., Gao, Z., Sun, X., Liu, C., Shangguan, D., Yang, W. and Gao, M. Protease-activated ratiometric fluorescent probe for pH mapping of malignant tumors. ACS nano, 9, 3199-3205 (2015).
[3] Ma, T., Hou, Y., Zeng, J., Liu, C., Zhang, P., Jing, L., Shangguan, D. and Gao, M. Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH in Vivo. J Am Chem Soc (2017).
n 鐩搁棞(gu膩n)鐢�(ch菐n)鍝�