纾烽吀鍖栨槸鏈�閲嶈銆佺爺绌舵渶寤ｆ硾鐨勪竴椤炶泲鐧借唱缈昏鍚庝慨椋惧舰寮�銆傛摎(j霉)绲�(t菕ng)瑷�锛岃秴閬庝笁鍒嗕箣浜岀殑浜洪铔嬬櫧璩彲浠ヨ纾烽吀鍖�锛岄�欎簺纾烽吀鍖栦綅榛炴鎴愮殑榫愬ぇ淇¤櫉缍�(w菐ng)绲＄偤浜哄�戣⿻鍒ょ窗鑳炵殑鐢熺悊鐙�鎱�(t脿i)銆侀憭瀹氱柧鐥呮蹇楃墿銆佺櫦(f膩)鐝�(xi脿n)钘ョ墿闈堕粸鎻愪緵浜嗛噸瑕佷俊鎭�傜敱浜庣７閰稿寲铔嬬櫧鐩稿皪浜庢櫘閫氳泲鐧借唱鑰岃█鍏锋湁浣庤睈搴﹀拰楂樺嫊鎱�(t脿i)鎬х殑鐗归粸锛屽洜姝ゅ皪纾烽吀鍖栬泲鐧借唱绲勫妯ｅ搧閫茶楂樼壒鐣版�у瘜闆嗘槸闄嶄綆妯ｅ搧寰╅洔绋嬪害銆佹彁楂樼７閰稿寲浣嶉粸閼掑畾鏁�(sh霉)鐩嚦闂滈噸瑕佺殑涓�姝�銆傚浐瀹氬寲閲戝爆闆㈠瓙瑕拰鑹茶瓬娉曪紙IMAC锛夋槸纾烽吀鍖栬泲鐧借唱绲勫鐮旂┒涓渶甯哥敤鐨勭７閰稿寲鑲芥瀵岄泦鎶�琛撲箣涓�銆�
鐧鹃潏濞佺崹瀹堕姺鍞敱涓闄㈠ぇ鍖栨墍鐢熺墿鍒嗛洟鍒嗘瀽鏂版潗鏂欒垏鏂版妧琛撶爺绌剁祫鍩轰簬姝ら枊鐧�(f膩)鍑虹殑鏂颁竴浠�纾烽吀鍖栬泲鐧借唱绲勫瀵岄泦鏉愭枡——Ti4+-IMAC[1]銆傚叾铔嬬櫧璩７閰稿寲閼掑畾鑳藉姏鏄父瑕�(gu墨)鍥哄畾鍖栭噾灞洟瀛愯Κ鍜岃壊璀滄硶锛�Fe3+-IMAC锛夌殑3鍊嶄互涓�锛岃垏TiO2鐩告瘮鍙互鎻愰珮绱�30%锛屽瘜闆嗙壒鐣版�у彲閬�95%[2]銆傚悓鏅�锛岃垏Zr-IMAC, ZrO2绛夌浉姣旓紝涔熷叿鏈夋洿鍎�(y艒u)鐣扮殑閬告搰鎬у拰瀵岄泦鏁堢巼[3][4]銆�
鍙冭�冩枃鐛�
[1] J. Proteome Res., 2008, 7, 3957
[2] J. Chromatogr. A, 2017, 1498, 22–28
[3] Nature Protocols, 2013, 8,3,461-480
[4] Molecular & Cellular Proteomics, 2011锛� 10:10.1074/mcp.M110.006452, 1–14
鈻� 鐢㈠搧鍒楄〃
鈻犲叾浠栫浉闂滅敘鍝佸垪琛�
|
鐢㈠搧绶ㄨ櫉
|
鐢㈠搧鍚嶇ū
|
CAS铏�
|
绱斿害
|
鍖呰瑕�(gu墨)鏍�
|
|
140105
|
1,4-Butanediol diglycidyl ether, 95%
1,4-涓佷簩閱囦簩绺按鐢樻补閱�
|
2425-79-8
|
95%
|
25g
100g
500g
|
|
314162
|
Sodium cyanoborohydride, 95%
姘板熀纭兼矮鍖栭垑
|
25895-60-7
|
95%
|
10g
50g
250g
|
|
551313
|
3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester, 96%
3-(2-鍚″暥浜岀～浠�)涓欓吀-N-鐞ョ弨閰颁簽鑳洪叝
|
68181-17-9
|
96%
|
100mg
|
|
308310
|
Streptavidin
閺堥湁瑕拰绱�
|
9013-20-1
|
N/A
|
5mg
25mg
|
|
249096
|
2-Mercaptoethanol, 99%, for electrophoresis and molecular biology
2-宸板熀涔欓唶
|
60-24-2
|
99%
|
10ml
250ml
|
|
191243
|
N,N'-Disuccinimidyl carbonate, 99%
N,N'-浜岀惀鐝�閰颁簽鑳哄熀纰抽吀閰�
|
74124-79-1
|
99%
|
5g
25g
100g
|
|
183144
|
N-Hydroxysulfosuccinimide sodium salt, 98%
N-缇ュ熀纾�?锛涱嫎顚剁壘顥嗗暫灏乧楣�
|
106627-54-7
|
98%
|
250mg
1g
5g
|
|
226162
|
Tris(hydroxymethyl)aminomethane, 99.5%, extra pure
涓�(缇ョ敳鍩�)姘ㄥ熀鐢茬兎
|
77-86-1
|
99.5%
|
25g
100g
500g
2.5kg
|
|
910322
|
mPEG-Amine, 95%, average M.W. 5,000
鐢叉哀鍩�-PEG-鑳�
|
80506-64-5
|
95%
|
1g
5g
|
|
932987
|
Amine-PEG-Amine, 95%, average M.W. 2,000
姘ㄥ熀-PEG-鑳�
|
24991-53-5
|
95%
|
1g
5g
|
鈻� 鐩搁棞鐢㈠搧