.jpg)
鎿�(j霉)绲�(t菕ng)瑷堬紝瓒呴亷涓夊垎涔嬩簩鐨勪汉椤炶泲鐧借唱鍙互琚７閰稿寲锛岄�欎簺纾烽吀鍖栦綅榛炴鎴愮殑榫愬ぇ淇¤櫉缍茬怠鐐轰汉鍊戣⿻鍒ょ窗鑳炵殑鐢熺悊鐙�鎱�(t脿i)銆侀憭瀹氱柧鐥呮蹇楃墿銆佺櫦(f膩)鐝�(xi脿n)钘ョ墿闈堕粸鎻愪緵浜嗛噸瑕佷俊鎭�傜７閰稿寲铔嬬櫧璩祫瀛告槸鐣朵笅铔嬬櫧璩爺绌朵腑闈炲父閲嶈鐨勬柟鍚�銆�
鐢变簬纾烽吀鍖栫殑铔嬬櫧璩浉灏嶄簬鏅�氳泲鐧藉叿鏈変綆璞愬害鍜岄珮鍕曟厠(t脿i)鎬х壒榛�锛屽洜姝ゅ皪纾烽吀鍖栬泲鐧借唱绲勫妯ｅ搧閫茶楂樼壒鐣版�у瘜闆�锛屾槸闄嶄綆妯ｅ搧寰╅洔绋嬪害銆佹彁楂樼７閰稿寲浣嶉粸閼掑畾鏁�(sh霉)鐩嚦闂滈噸瑕佺殑涓�姝ャ�傚叾涓�锛屽浐瀹氬寲閲戝爆闆㈠瓙瑕拰鑹茶瓬娉曪紙IMAC锛夋槸纾烽吀鍖栬泲鐧借唱绲勫鐮旂┒涓渶甯哥敤鐨勭７閰稿寲鑲芥瀵岄泦鎶�琛撲箣涓�銆�
鐧鹃潏濞佹敎鎵嬩腑绉戦櫌澶у寲鎵�钁夋槑浜暀鎺�锛屾彁渚涙柊涓�浠ｇ７閰歌偨瀵岄泦鏉愭枡——Ti-IMAC[1]锛岀敱浜庡叾鍗撹秺鐨勫瘜闆嗘�ц兘锛屽湪鍦嬪収澶栫嵅寰椾簡寤ｆ硾闂滄敞銆傜洰鍓�锛屽凡鏈�70澶氬�嬪湅鍏у鐭ュ悕楂樻牎銆佺鐮旈櫌鎵�銆佺敓鐗╃鎶�鍏徃浣跨敤鎴戝徃Ti-IMAC鏉愭枡銆�Ti-IMAC鏉愭枡琚噳鐢ㄤ簬浜鸿倽纾烽吀鍖栬泲鐧借唱绲勭殑瑕�(gu墨)?锛涱嚞娌�?锛屽緱鍒颁簡浜鸿倽绲勭箶鏈�澶х殑纾烽吀鍖栦綅榛炴暩(sh霉)鎿�(j霉)闆嗭紙涓嬪湒1锛�銆�[2]
鈻� 鐢㈠搧鐗规��
- 瓒呴珮鐨勮泲鐧借唱纾烽吀鍖栭憭瀹氳兘鍔涳細鏄父瑕�(gu墨)鍥哄畾鍖栭噾灞洟瀛愯Κ鍜岃壊璀滄硶锛�Fe3+-IMAC锛夌殑3鍊嶄互涓�锛岃垏TiO2鐩告瘮鍙互鎻愰珮绱�30%锛屽瘜闆嗙壒鐣版�у彲閬�95%[3]锛�
- 鏇撮珮鐨勭７閰歌偨瀵岄泦鏁堢巼锛氳嵎铇湅瀹惰泲鐧借唱绲勫鐮旂┒涓績鐨�Heck鏁欐巿瀵﹂瀹ゅ皥闁�灏嶈┎鏉愭枡閫茶浜嗘脯瑭︼紝绲愭灉琛ㄦ槑鑸囧父瑕�(gu墨)鐨勭７閰歌偨瀵岄泦鏉愭枡濡�TiO2鐩告瘮锛�Ti-IMAC鍏锋湁鏇撮珮鐨勫瘜闆嗘晥鐜�[4]锛�
- 鐛ㄥ鐝�(xi脿n)璨ㄤ緵鎳�銆�
鍦�1 Ti-IMAC琚噳鐢ㄤ簬浜鸿倽纾烽吀鍖栬泲鐧借唱绲勭殑瑕�(gu墨)妯″寲鍒嗘瀽锛屽緱鍒颁簡浜鸿倽绲勭箶鏈�澶х殑纾烽吀鍖栦綅榛炴暩(sh霉)鎿�(j霉)闆�[2]
鈻� 鐢㈠搧鍒楄〃
鈻� 鍏朵粬鐩搁棞鐢㈠搧
|
鐢㈠搧绶ㄨ櫉
|
鐢㈠搧鍚嶇ū
|
CAS铏�
|
绱斿害
|
鍖呰瑕�(gu墨)鏍�
|
|
140105
|
1,4-Butanediol diglycidyl ether, 95%
1,4-涓佷簩閱囦簩绺按鐢樻补閱�
|
2425-79-8
|
95%
|
25g
100g
500g
|
|
970232
|
4-Aminobenzamidine dihydrochloride
4-姘ㄥ熀鑻敳鑴掍簩楣介吀楣�,瓒呯磾98%
|
2498-50-2
|
98%
|
1g
5g
25g
100g
|
|
314162
|
Sodium cyanoborohydride, 95%
姘板熀纭兼矮鍖栭垑
|
25895-60-7
|
95%
|
10g
50g
250g
|
|
551313
|
3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester
3-(2-鍚″暥浜岀～浠�)涓欓吀-N-鐞ョ弨閰颁簽鑳洪叝
|
68181-17-9
|
96%
|
100mg
|
|
308310
|
Streptavidin
閺堥湁瑕拰绱�
|
9013-20-1
|
N/A
|
5mg
25mg
|
|
249096
|
2-Mercaptoethanol, 99%, for electrophoresis and molecular biology
2-宸板熀涔欓唶
|
60-24-2
|
99%
|
10ml
250ml
|
|
191243
|
N,N'-Disuccinimidyl carbonate, 99%
N,N'-浜岀惀鐝�閰颁簽鑳哄熀纰抽吀閰�
|
74124-79-1
|
99%
|
5g
25g
100g
|
|
183144
|
N-Hydroxysulfosuccinimide sodium salt, 98%
N-缇ュ熀纾�?锛涱嫎顚剁壘顥嗗暫灏乧楣�
|
106627-54-7
|
98%
|
250mg
1g
5g
|
|
226162
|
Tris(hydroxymethyl)aminomethane
涓�(缇ョ敳鍩�)姘ㄥ熀鐢茬兎锛岃秴绱�
|
77-86-1
|
99.5%
|
25g
100g
500g
2.5kg
|
|
910322
|
mPEG-Amine, 95%, average M.W. 5,000
鐢叉哀鍩�-PEG-鑳�
|
80506-64-5
|
95%
|
1g
5g
|
|
932987
|
Amine-PEG-Amine, 95%, average M.W. 2,000
姘ㄥ熀-PEG-鑳�
|
24991-53-5
|
95%
|
1g
5g
|
鈻� 鐩搁棞鐢㈠搧
鈻�鍙冭�冩枃鐛�
[1] Zhou H, Ye M, Dong J, Han G, Jiang X, Wu R, Han F. Journal of Proteome Research. 2008, 7, 3957-3967.
[2] Bian Y, et al. J. Proteomics. 2014, 96, 253-262.
[3] Yao Y, Dong J, Dong M, Liu F, Wang Y, Mao J, Ye M, Zou H. J. Chromatogr. A. 2017, 1498, 22–28.
[4] Zhou, Houjiang,Low, Teck Y.,Hennrich, Marco L.,et al. Molecular & Cellular Proteomics. 2011,10(10):1.
鈻� 閰嶅瑭﹀姂瑾槑1
銆愮敘鍝佺法铏熴��2749380
銆愯嫳鏂囧悕绋便��CAE-Ti-IMAC, 100%
銆愪腑鏂囧悕绋便�戝柈鍒嗘暎鍥哄畾鍖栬Κ鍜岃壊璀滃井鐞�
銆愮敘鍝佺啊浠嬨�戞湰鐢㈠搧閬╃敤浜庡皪纾烽吀鑲界殑閼掑畾鍜屽瘜闆�銆�
銆愪娇鐢ㄦ鐢㈠搧鎵�闇�瑭﹀姂鍒楄〃銆�
|
瑭﹀姂鍒楄〃
|
涓枃鍚嶇ū
|
鎵�闇�婧惰唱
|
CAS
|
灏嶆噳鎴戝徃鐢㈠搧绶ㄨ櫉
|
|
Ti(SO4)2
|
纭吀閳�
|
纭吀閳�
|
13693-11-3
|
鐒�
|
|
0.1% TFA姘存憾娑�
|
0.1%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
200mM NaCl姘存憾娑�
|
200mM 姘寲閳夋按婧舵恫
|
姘寲閳�
|
7647-14-5
|
105864
|
|
30% H2O2
|
30% 闆欐哀姘�
|
30% 闆欐哀姘�
|
7722-84-1
|
鐒�
|
|
80%ACN
|
80%涔欒厛
|
涔欒厛
|
75-05-8
|
168171
|
|
6% TFA姘存憾娑�
|
6%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
50%ACN
|
50%涔欒厛
|
涔欒厛
|
75-05-8
|
168171
|
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
7664-41-7
|
鐒�
|
|
1%FA姘存憾娑�
|
1%鐢查吀姘存憾娑�
|
鐢查吀
|
64-18-6
|
949722
|
鈻� 閰嶅瑭﹀姂瑾槑2
銆愮敘鍝佺法铏熴��2749381
銆愯嫳鏂囧悕绋便��SPE-Ti-IMAC, 100%
銆愪腑鏂囧悕绋便�戝浐鐩歌悆鍙栧浐瀹氬寲瑕拰鑹茶瓬寰悆
銆愪繚瀛樻浠躲�戝婧劜瀛�锛屼繚璩湡鐐轰簲骞�銆�
銆愮敘鍝佺啊浠嬨�戞湰鐢㈠搧閬╃敤浜庡皪纾烽吀鑲界殑閼掑畾鍜屽瘜闆嗐��
銆愪娇鐢ㄦ鐢㈠搧鎵�闇�婧跺姂鍙婅�楁潗鍒楄〃銆�
|
瑭﹀姂鍒楄〃
|
涓枃鍚嶇ū
|
鎵�闇�婧惰唱
|
CAS
|
灏嶆噳鎴戝徃鐢㈠搧绶ㄨ櫉
|
|
Ti(SO4)2
|
纭吀閳�
|
纭吀閳�
|
13693-11-3
|
鐒�
|
|
0.1% TFA姘存憾娑�
|
0.1% 涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
200mM NaCl姘存憾娑�
|
200mM 姘寲閳夋按婧舵恫
|
姘寲閳�
|
7647-14-5
|
105864
|
|
30% H2O2
|
30% 闆欐哀姘�
|
30% 闆欐哀姘�
|
7722-84-1
|
鐒�
|
|
80%ACN
|
80%涔欒厛
|
涔欒厛
|
75-05-8
|
168171
|
|
6% TFA姘存憾娑�
|
6%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
50%ACN
|
50%涔欒厛
|
50%涔欒厛
|
75-05-8
|
168171
|
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
7664-41-7
|
鐒�
|
|
1%FA姘存憾娑�
|
1%鐢查吀姘存憾娑�
|
鐢查吀
|
64-18-6
|
949722
|
|
200 μL妲嶉牠
|
绉绘恫鍣ㄥ惛闋�
|
/
|
/
|
970956
|
|
600 ul闆㈠績绠�
|
闆㈠績绠�
|
/
|
/
|
910854
|
|
1.5 ml闆㈠績绠�
|
闆㈠績绠�
|
/
|
/
|
915664
|
|
SPE鏌辩鍙婄鏉�
|
绡╂澘
|
/
|
/
|
1ml锛�2579375
3ml锛�961915
|