銆愮敘(ch菐n)鍝佺法铏熴��2793950
銆愯嫳鏂囧悕绋便��Locus-specific, quantitative 5fC analysis Kit
銆愪腑鏂囧悕绋便��鐗瑰畾浣嶉粸5-閱涘熀鑳炲槯鍟跺畾閲忔娓│鍔戠洅
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鐢�(ch菐n)鍝佺祫鎴�
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鍚噺
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婧舵恫L1锛�Buffer L1锛�
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2.4 mL
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M
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24绠�
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妯″紡搴忓垪
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24ul
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娉細婧舵恫L1锛氫笝浜岃厛鍙嶆噳娑诧紱M锛氫笝浜岃厛
銆愪繚瀛樻浠躲��妯″紡搴忓垪缃簬-20 °C锛屽彲淇濆瓨12鍊嬫湀銆�
M鍜屾憾娑�L1缃簬4 °C锛屽彲淇濆瓨12鍊嬫湀銆�
M闇�閬垮厜淇濆瓨銆�
銆愮敘(ch菐n)鍝佹杩般��
5-鐢插熀鑳炲槯鍟朵慨椋撅紙5mC锛夋槸鐪熸牳绱拌優鍩哄洜绲勪腑鏈�鐐洪噸瑕佺殑琛ㄨ閬哄偝淇＞涔嬩竴銆�5mC鍙互鍦�TET瀹舵棌閰剁殑浣滅敤涓嬮�愭姘у寲鐢熸垚5-缇ョ敳鍩鸿優鍢у暥锛�5hmC锛�銆�5-閱涘熀鑳炲槯鍟讹紙5fC锛夊拰5-缇у熀鑳炲槯鍟讹紙5caC锛�锛涘皪浜庝笂杩板咕绋柊鍨�DNA淇＞鐨勫姛鑳界爺绌舵槸鐩墠鏍搁吀琛ㄨ閬哄偝瀛哥殑涓�鍊嬬爺绌剁啽榛�銆�
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• Oligo Clean & Concentrator (ZYMO, #D4060)
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DNA绱斿害瑕佹眰锛�OD260锛�OD280锛�1.8锝�2.0
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