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PI3-K瀹舵棌鍙冭垏澶氱ó淇¤櫉閫氳矾锛岃(di脿o)绡�(ji茅)绱�(x矛)鑳炵殑涓昏鍔熻兘銆傛甯告儏娉佷笅锛岀敱鍏舵椿鍖栬�岀敘(ch菐n)鐢熺殑椤炶剛鐢�(ch菐n)鐗PI(3,4)P2]鍜孾PI(3,4,5)P3]浣滅偤绗簩淇′娇绲�(ji茅)鍚堝苟婵�娲诲绋窗(x矛)鑳炲収(n猫i)鐨勯澏铔嬬櫧锛屽舰鎴愪竴鍊嬩俊铏熺礆鑱�(li谩n)寰�(f霉)鍚堢墿锛屾渶绲傝(di脿o)绡�(ji茅)绱�(x矛)鑳炵殑澧炲��銆佸垎鍖�銆佸瓨娲诲拰閬风Щ绛�銆�
Class鈪燩I3-K鏄竴鍊嬩簩鑱氶珨铔嬬櫧锛屽叿鏈夐鑴傛縺閰跺拰铔嬬櫧婵�閰剁殑闆欓噸娲绘��銆傛縺娲诲悗鐨凱I3-K鑳藉湪璩�(zh矛)鑶滀笂浣縖PI(3,4)P2]纾烽吀鍖栨垚[PI(3,4,5)P3]锛� [PI(3,4,5)P3]鍙皫(d菐o)鑷碅kt鐨勬椿鍖�銆傛椿鍖栫殑Akt閫氶亷纾烽吀鍖栦綔鐢ㄦ縺娲绘垨鎶戝埗鍏朵笅娓哥殑Bad銆丆aspase9銆丯F-kB銆丟SK-3銆丗KHR銆乸21銆乸27绛夐澏铔嬬櫧锛岄��(j矛n)鑰岃(di脿o)绡�(ji茅)绱�(x矛)鑳炵殑澧炲��銆佸垎鍖�銆佸噵浜′互鍙婇伔绉�銆傚洜鑰孭I3-K鍜孾PI(3,4,5)P3]宸茬稉(j墨ng)鎴愮偤灏嶅寘鎷値鐧ャ�佺檶鐧ョ瓑澶氱ó鐤剧梾閫�(j矛n)琛岀爺绌跺拰钘ョ墿瑷�(sh猫)瑷�(j矛)鐨勯澏鐩镐綅榛�(di菐n)銆�
鈽呯敘(ch菐n)鍝佸劒(y艒u)榛�(di菐n)
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鐢�(ch菐n)鍝佺法铏�
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鍚嶇ū
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妾㈡脯鏂规硶
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PI3-K ELISA锛歅ico Assay
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ELISA娉�
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1 kit
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PI3-Kinase Fluorescence Polarization Kit, 384 assays
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鐔掑厜鍋忔尟娉�(FP)
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1 kit
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ClassIII PI3-K ELISA Kit
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ELISA娉�
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1 kit
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PI3-K AlphaScreen®
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AlphaScreen鎶�琛�(sh霉)
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1 kit
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1銆丳I3-K閰惰伅(li谩n)鍏嶇柅娓畾瑭﹀姂鐩掞紙PI3-K ELISA锛歅ico Assay锛孠-1000s锛�
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2銆佹噳(y墨ng)鐢ㄤ簬AlphaScreen鎶�琛�(sh霉)涓婄殑PI3-K妾㈡脯瑭﹀姂鐩�
AlphaScreen鍒╃敤浣滅偤渚涢珨鍜屽彈楂旂殑寰彔渚嗘娓敓鐗╁垎瀛愮殑鐩镐簰浣滅敤锛屽井鐝犺〃闈㈣钃嬩簡涓�灞ゆ按鍑濊啝锛屼綔鐐虹敓鐗╅�ｆ帴鐨勫姛鑳藉熀鍦�(tu谩n)銆傜暥(d膩ng)鐢熺墿鍒嗗瓙瀛樺湪鐩镐簰浣滅敤鏅傦紝閫欑ó鐩镐簰浣滅敤鏈冨皣渚涢珨鍜屽彈楂斿井鐝犳媺杩�锛屽緸鑰屾縺鐧�(f膩)绱氳伅(li谩n)鏀惧ぇ鐨勫寲瀛�(xu茅)鍙嶆噳(y墨ng)锛岀敘(ch菐n)鐢熸サ澶у寮�(qi谩ng)浜嗙殑淇¤櫉銆�
鎳�(y墨ng)鐢ˋlphaScreen鎶�琛�(sh霉)鐨凱I3-K妾㈡脯瑭﹀姂鐩掓渻鏈夋洿澶氬劒(y艒u)鍕細
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3銆丳I3-K鐔掑厜鍋忔尟娓畾瑭﹀姂鐩掞紙PI 3-Kinase Fluorescence Polarization Kit, 384 assays锛孠-1100锛�
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4銆丆lass III PI3-K閰惰伅(li谩n)鍏嶇柅娓畾瑭﹀姂鐩掞紙Class III PI3-K ELISA Kit锛孠-3000锛�
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