鈻� 鏄庢槦鐢�(ch菐n)鍝�——鐠�(hu谩n)澧冨弸濂界殑鍗佷簩鐑峰熀纭吀閳�(SDS)
 鍗佷簩鐑峰熀纭吀閳変篃绋辩偤鏈堟鍩虹～閰搁垑锛堢啊绋�SDS锛�锛屽湪DNA鎻愬彇閬庣▼涓紝鑳戒娇铔嬬櫧璩�(zh矛)璁婃�у悗鑸�DNA鍒嗛枊锛屽父鐢ㄤ簬鐢熷寲銆佸厤鐤椹�銆�SDS灏嶇矘鑶�銆佷笂鍛煎惛閬撱�佺溂鍙婄毊鑶氭湁鍒烘縺浣滅敤锛屽彲寮曡捣鍛煎惛绯荤当(t菕ng)閬庢晱鎬у弽鎳�(y墨ng)銆�- 鐧鹃潏濞佺殑鏂扮⿴鍨嬮绮掔媭SDS鏈韩涓嶆渻鐢�(ch菐n)鐢熺矇濉�锛屽緸鏍规湰涓婃潨绲曚簡SDS绮夊〉鐨勪竴鍒囧嵄瀹筹紝鏄竴绋挵(hu谩n)澧冨弸濂藉瀷SDS銆�
- 鍏惰唱(zh矛)閲忓拰绌�(w臎n)瀹氱殑搴瓨鑳藉婊胯冻鐢熺墿鍖栧(xu茅)銆佸垎瀛愮敓鐗╁(xu茅)绛夌爺绌堕牁(l菒ng)鍩熺殑闇�姹�銆�
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鐢�(ch菐n)鍝佺法铏�
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鑻辨枃鍚嶇ū
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涓枃鍚嶇ū
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CAS
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瑕�(gu墨)鏍�
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Dodecylsulfate-Na-salt in pellets (SDS), 99%
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鍗佷簩鐑峰熀纭吀閳夛紙椤嗙矑鐙�锛�
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250 g
1 kg
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鈻� SDS-PAGE鐩搁棞(gu膩n)瑭﹀姂鍜岀珐娌栨恫
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绶╂矕娑�
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鎴愬垎
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鐢�(ch菐n)鍝佺法铏�
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閰嶅埗鏂规硶
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30%(W/V)涓欑儻閰拌兒
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涓欑儻閰拌兒
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1. 绋卞彇290 g涓欑儻閰拌兒锛�10 g BIS锛屽姞鍏�600 mL鍘婚洟瀛愭按锛屽厖鍒嗘敧鎷屾憾瑙�锛�
2. 鍔犲叆鍘婚洟瀛愭按瀹氬鑷�1 L锛岀敤0.45 μm婵捐啘婵惧幓闆滆唱(zh矛)锛屼簬妫曡壊鐡�4鈩冧繚瀛樸��
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N,N-浜炵敳鍩洪洐涓欑█閰拌兒
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10%(W/V)閬庣～閰搁姩
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閬庣～閰搁姩
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绋卞彇1 g閬庣～閰搁姩锛屽姞鍏�10 mL 鍘婚洟瀛愭按鏀媽婧惰В锛岃帛瀛樹簬4鈩�
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5×Tris-Glycine Buffer
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Tris
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1. 绋卞彇15.1 g Tris锛�94 g鐢樻皑閰稿拰5.0 g SDS锛屾憾浜�800 mL鍘婚洟瀛愭按锛�
2. 鍔犲幓闆㈠瓙姘村畾瀹硅嚦1 L锛屽婧繚瀛樸��
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鐢樻皑閰�
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SDS
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5×SDS-PAGE Loading Buffer
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Tris-HCl
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1. 绋卞彇1.25 mL鐨�1 M Tris-HCl锛�0.5 g SDS锛�25 mg BPB鍜�2.5 mL鐢樻补锛屽姞鍘婚洟瀛愭按婧惰В锛�
2. 鍘婚洟瀛愭按瀹氬鑷�5 mL锛�500 μl/浠藉垎瑁濆悗锛屽婧繚瀛�銆�
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SDS
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2076502 |
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婧撮厷钘�
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鐢樻补
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鏌撹壊娑�
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鑰冮Μ鏂寒钘峈-250
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1. 绋卞彇1 g鑰冮Μ鏂寒钘峈-250锛屽姞鍏�250 mL鐣颁笝閱�锛屾敧鎷屾憾瑙�锛�
2. 鍔犲叆100 mL鍐伴唻閰稿拰650 mL鍘婚洟瀛愭按锛屽潎鍕绘敧鎷�锛�
3. 鐢ㄦ烤绱欐烤闄ら绮掔墿璩�(zh矛)鍚庯紝瀹ゆ韩淇濆瓨銆�
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鐣颁笝閱�
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涔欓吀
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鑴壊娑�
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涔欓吀
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绋卞彇100 mL閱嬮吀锛�50 mL涔欓唶鍜�850 mL鍘婚洟瀛愭按锛屽厖鍒嗘贩鍕汇��
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涔欓唶
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鈻� 甯哥敤鐢熷寲瑭﹀姂
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鐢�(ch菐n)鍝佺法铏�
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鑻辨枃鍚嶇ū
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涓枃鍚嶇ū
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CAS
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瑕�(gu墨)鏍�
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EB, 95%
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婧村寲涔欏暥
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1 g
5 g
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Imidazole, 99%
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鍜攽
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500 g
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TEMED, 99%
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N,N,N',N'-鍥涚敳鍩轰箼浜岃兒
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100 mL
500 mL
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DTT, 99%
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浜岀～铇囩硸閱�
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1 g
5 g
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Tween 80
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鍚愭韩80
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25 mL
250 mL
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PMSF, 99%
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鑻敳鍩虹：閰版盁
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5 g
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PEG 6000
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鑱氫箼浜岄唶
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250 g
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Albumin, 98%, from bovine serum: BSA
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鐗涜娓呯櫧铔嬬櫧锛屼締婧愪簬鐗涜娓�
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5 g
25 g
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IPTG
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鐣颁笝鍩�-β-D-纭唬鍗婁钩绯栧悺鍠冪硸鑻�
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25 g
100 g
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X-gal
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5-婧�-4-姘�-3-鍚插摎锛�β-鍗婁钩绯栬嫹
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1 g
5 g
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PVP
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鑱氫箼鐑悺鍜兎閰�
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500 g
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DEPC, 97%
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鐒︾⒊閰镐簩涔欓叝
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25 mL
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绱拌優鍩归(y菐ng)鐩搁棞(gu膩n)瑭﹀姂
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